Advances in Fluorescence Microscopy
نویسندگان
چکیده
منابع مشابه
Recent Advances in Fluorescent Labeling Techniques for Fluorescence Microscopy
Tremendous progress in recent computer-controlled systems for fluorescence and laser-confocal microscopy has provided us with powerful tools to visualize and analyze molecular events in the cells. Various fluorescent staining and labeling techniques have also been developed to be used with these powerful instruments. Fluorescent proteins such as green fluorescent protein (GFP) allow us to direc...
متن کاملFluorescence and fluorescence microscopy
The concept of specific cellular antigen staining by use of an immunofluorescent technique was introduced by AH COONS and co-workers about 60 years ago. Fluorescent probes are efficient tools and enable the detection of particular components in complex structures of organs including live cells. Under the condition of specific molecular interactions, fluorochrome labeled ligands allow the select...
متن کاملFluorescence Microscopy
When organic or inorganic specimens absorb and subsequently reradiate light, the process is typically a result of fluorescence or phosphorescence. Fluorescence emission is nearly simultaneous with the absorption of the excitation light as the time delay between photon absorption and emission is typically less than a microsecond. When the emission persists long after the excitation light is exti...
متن کاملFrontiers in fluorescence microscopy.
How we see organisms and cells depends on the tools at our disposal. For over 150 years, biologists were forced to rely on fixed, dehydrated and stained specimens in order to guess how the living cells could function. It all changed abruptly during the last two decades when the rapid development of novel imaging techniques revolutionized the way scientists look at the structures of life alive.
متن کاملFluorescence Microscopy
The human eye requires contrast to perceive details of objects. Several ingenious methods of improving the contrast of microscopy images have been designed, and each of them opened new applications of optical microscopy in biology. The simplest and very effective contrasting method is ‘‘dark field.’’ It exploits the scattering of light on small particles that differ from their environment in re...
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ژورنال
عنوان ژورنال: Optik & Photonik
سال: 2007
ISSN: 1863-1460
DOI: 10.1002/opph.201190254